## To generate Summary_24h.tsv and Summary_48h.tsv
source run_all.sh

## Folder information
0.Primer_information
All information about feature genes list(Feature_genes_list.xlsx), sequences of PCR primers (cDNA_seqence folder and Primers_datails.xlsx), and check PCR products (Feature_genes_primer_check.pptx).And memo for Feature_genes_primer_check.pptx.txt are added.

1.StepOnePlus_raw_output
These files were exported from StepOnePlus (ThermoFisher Scientific) in txt format.
24hrs_redo and 48hrs_redo files are used for undetected samples or a little low quality samples.However a little law quality samples are not influenced CT directly after evaluation.

2.Redo_files
These files were used for final data.

3.Sample_name_for_selected_data
These files were all sample name files correspond to 1.StepOnePlus_raw_output files.
# 24hrs_redo/20210521_re_24hr_56Tm2_data_sample_name.txt
Changed name "Sample 60" to "Sample 72" because we made a mistake at StepOnePlus execution.

4.Final_data
These files were combined from 1. to 3. 

